Subnormothermic ex vivo lung perfusion attenuates ischemia reperfusion injury from donation after circulatory death donors

Use of normothermic ex vivo lung perfusion (EVLP) was adopted in clinical practice to assess the quality marginal donor lungs. Subnormothermic temperatures are use among other solid organs improve biochemical, and immunological parameters. In a rat EVLP model donation after circulatory death (DCD) donors, we tested effect four subnormothermic that could further organ preservation. Warm ischemic time 2 hours. 4 Lung physiological data were recorded metabolic parameters assessed. oxygenation at 21°C 24°C significantly improved whereas pulmonary vascular resistance edema formation worsened when compared 37°C EVLP. The perfusate concentrations potassium ions lactate exiting lungs with 28°C lower sodium chlorine 32°C higher Also EVLP, pro-inflammatory chemokines MIP2, MIP-1?, GRO-?, cytokine IL-6 21°C, IL-18 but only IL-1? Compared tissue ATP content higher, carbonylated protein measured myeloperoxidase activities tissues 32°C. demonstrated acceptable variables, decreased proteins reduced release cytokines. conclusion, is non inferior setting comparison clinically approved may reduce I/R injuries DCD donors.